This lab, we were testing the effect of temperature

This lab was divided into four different parts and as a group we established different hypothesis for each part of the lab. In the first part of the lab, we were testing whether catalase is reusable or not, thus we hypothesized that the liver tissue is reusable and would react but not as much as if you were to put more hydrogen peroxide 3% in the enzyme liquid. For part B of the lab, we were observing which tissues, from potato, apple and chicken  contain catalase. We hypothesized for this part of the lab, that the three tissues will contain catalase  but with different rate of reaction, if it immersed in hydrogen peroxide 3% solution. In the third part of the lab, we were testing the effect of temperature on catalase activity by immersing liver tissues in three different temperature. Therefore, we hypothesized for this portion of the lab, that the liver tissue will react more efficiently and properly, if it was immersed in a temperature close to the room temperature. While if it was immersed in temperature far from the room temperature, then the liver tissue wont react efficiently and will have a low rate of reaction as it will  creates the catalase to break down or denature and not work properly.  In the final part of the lab, we were observing the effect of different pH levels on catalase activity. Therefore, we hypothesized that the liver tissue will work more efficiently and probably, if it was immersed in a basic solution. while  if it was immersed in an acidic solution enzyme will  face complete loss of activity . Even though  our experiment contained many flaw, the collected data did support the hypothesis for a high extent.In the first part of the lab, the rate of the reaction of the liver added to use peroxide was 0 as The liquid after the reaction is just H2O, so nothing happens when a new piece of liver is added to the used hydrogen peroxide and this was seen as there was no bubbles. However when more hydrogen peroxide were added to the used liver tissue, the rate of the reaction were back to 5 again. This fully supported my hypothesis, which states that enzymes catalase is reusable, if more hydrogen peroxide is added to it.  From this, we knew that catalase was reusable, since catalase itself is not altered by the breaking down of chemicals, It just  is  involved in the decomposition of hydrogen peroxide (H2O2H2O2) into hydrogen and water. Therefore we could add more hydrogen peroxide to the liver and there would still be a reaction. . On the contrary, we could not add new liver to the  liquid that had already been diluted because it is just water.In the second part of the lab, all the tissue from potato, apple and chicken contain catalase, as they all had a rate of reaction but some contained more. The chicken contain more catalase as it had the fastest reaction rate 5, while the test tubes of the apple had the slowest reaction 1, since it had few bubbles fizzling out from the bottom of the test tube. This fully supported my hypothesis which state, that enzymes contain catalase, as all the tissues did have a reaction but with different rates when we added the 2 ml of hydrogen peroxide. Catalase is a significant enzyme and is found in all tissue as it doesn’t  only allows the organism to maintain homeostasis, but also it breaks down toxins that are made as a product of metabolism and transforms that harmful substance into essential things for life.In part C of the lab, the warm liver tissue reacts fast with reaction rate 5 as there was an immediate response of oxygen fizzling from the bottom of the test tube in the forms of bubbles. While the boiled liver tissue had no reaction, reaction rate 0, since there were no bubbles fizzing from the bottom of the test tubes. This fully supported my hypothesis which states that enzyme will work at its peak with a temperature near to the room temperature and that if it was immersed in temperature far from the room temperature, and then the liver tissue won’t react efficiently, which shows that the temperature have an effect on the rate of the enzyme activity. As if the temperature is higher or lower than the optimum temperature ( the room temperature) as higher temperature will cause enzyme stability to decrease to decrease as the thermal energy disrupts the hydrogen bonds holding the enzyme together causing the enzyme to denature ( lose its shape) and not work properly. Whereas low temperature will result in insufficient thermal energy for the activation to take place.In the last portion of the lab, the enzyme worked at its peak in the basic solution pH 10 as it has a rate of reaction, while in the acidic solution the rate of the reaction was 1. By this way, my hypothesis was supported, as it states that the liver tissue will work more efficiently and probably, if it was immersed in a basic solution and this shows that the pH has an effect on the rate of the enzyme activity. Enzymes have an optimum temperature, which seems to be pH 10 for the liver tissue, and moving outside this optimum will cause the enzyme to lose its solubility and shape, diminishing the rate of the reaction.  In part C and D,  a pattern were shown between the level of temperature and pH and the rate of the reaction. As when  the temperature or the pH level increases, the rate of the reaction increases. Evaluation:Further investigations can include qualitative data such as timed speeds of the reaction as well as calculating the mass before and after the reactions to note the difference, if a difference did occur.Further experimentation is needed to fully determine the exact temperature and pH range in which catalase can be fully utilized. Error/LimitationSignificanceImprovementThe amount of trails of each experiments The rate of the reaction may not have been accurate first because , each group including mine had two or more participant deciding on the rate of the reaction. Moreover,  each step was not repeated multiple of times to ensure precision instead it was only tested once. Test each part of the of the experiment couple of time to ensure the precision of the rate as we usually have different opinion and this will helps ensure the right rate. The size of the pieces of the tissue Another thing that might have been a factor to add to the lab being inaccurate is that not all the liver and chicken pieces were cut up the same way. This could have had an affect on the rate of reaction as the bigger the piece, the more substrate concentration : This could be avoided if the pieces were all weighed before they were placed in the solutions. The equipments used in this experiment were not calibrated and additionally some test tubes had some water droplets inside them. The fact that the test tubes contained some water droplets on them may have affected the enzyme activity rate as it could have been a physical interference between the enzyme and substrate molecules and thus it will diminish the rate of the enzyme activity. However, this is a minor error and it is only slightly significant since the first the small amount of water can hardly affect the large amount of solution All the equipment’s that were used in the experiment must be calibrated before the experiment to ensure that there is no other uncontrolled variables can affect the rate of the reaction. This will increase precision, accuracy, and reliability of data. Furthermore, the test tube must be dried off using paper towel before pouring the solution in the test tube.

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